Solid Phase Peptide Synthesis Equipment (SPPS)

Büchi Glas Uster - Solid Phase Synthesis Equipment

Büchi AG in Uster Switzerland is a leading manufacturer of pilot-plant and pressure reactors for the chemical and pharmaceutical industry. The plants and reactors made from glass and metal are exported to over 40 countries worldwide.

Technical Features:

General features

  • Glass filter vessel 10 – 300 liter
  • -1.0 up to 0.5 bar
  • -60 °C to 200 °C
  • chemical inert materials: glass / PTFE / PFA / ETFE
  • cGMP design / complies with FDA regulations
  • ATEX / MRL

Standard issue features

  • Coated cover plate
  • Dry running mechanical seal
  • Various mesh sizes, filter mesh 8 – 115 μm / support plate
  • Lift for easy opening / discharge and cleaning / filter change
  • Stainless steel scaffolding

Customizations for solid phase reactors

  • Manually or motor driven stirrers
  • Stirrer design in different shapes, custom according to specifications
  • Free nozzle arrangement on cover plate
  • Spray nozzles CIP for cleaning or solvent addition
  • Inlet tubes in various shapes and designs
  • Level switches for overflow protection or automation
  • Liquid feeding batteries for solvents, reactants, others  
  • Load cell - weighing system
  • Heating / cooling jacket  
  • Adaptation for high containment powder transfer systems
  • Design materials: stainless steel 316L, Hastelloy® C22,
    glass lined steel, PTFE, PFA – coated
  • Glass overhead, e.g. for drying
  • Automation control system
  • Connection interfaces
Fully automated 100 lt. peptide synthesizer with load cells, feeding batteries and all options.
Fully automated 100 lt. peptide synthesizer with load cells, feeding batteries and all options.
Solid phase reactor 10 lt. with heating/cooling jacket, motorized anchor stirrer, interchangeable stirrer design.
Solid phase reactor 10 lt. with heating/cooling jacket, motorized anchor stirrer, interchangeable stirrer design.
Solid phase 30 lt. tiltable, manual stirrer height adjustable.
Solid phase 30 lt. tiltable, manual stirrer height adjustable.
Solid phase 50 lt., with lift and swing out design, 2 stage stirrer with fixed height.
Solid phase 50 lt., with lift and swing out design, 2 stage stirrer with fixed height.
Fully automated 100 lt. peptide synthesizer with load cells, feeding batteries and all options.
Fully automated 100 lt. peptide synthesizer with load cells, feeding batteries and all options.

The main advantages of our equipment:

Quality
Buchi glass equipment offers superior performance for R&D, pilot plants, technical centers, kilo lab and production for more than 75 years. Swiss quality workmanship guarantees dependable operation and a long life of reliable service.


Safety
Buchi reactors comply with the most stringent international safety standards such as EC Machinery Directive, ATEX, TA Luft, PED and Directive 2003/94/EC and 1991/412/EEC, Part I, Chapter 3 and annex, part 11.


Customization
Our strength lies in custom made conceptual design and development of equipment for a variety of customer needs and applications. We offer everything from a single source: complete software solutions, engineering and procurement of components.

A selection of possible applications that can be performed with Buchi equipment:

procedure reagent LPPS SPPS
mixed anhydride coupling CI-forminate, NMM √  
DCC coupling DCC, HOBt, NMM, HBTU √ √
Azo coupling Hydrazine, tBu-nitrite, Amine √  
Z- protection group Z-Cl, NaOH √  
Z- cleavage H2, Pd catalyst √  
Boc- protection group Boc-anhydride, NaOH √ √
Boc- cleavage Trifouoracetic acid √ √
Fmoc- protection group Fmoc - OSu, Na2CO3 √ √
Fmoc- cleavage piperidine √ √
Acylation Acetylchloride √ √
Guanidium group protection Tos- , Mtr-, Pmc-, Pbf- √  
-SH group protection Acm- √  
Esterification OEt, OtBu-, OBzl- √  

What is Solid-phase synthesis?

Solid-phase synthesis is the most common technique for peptide synthesis. Usually, peptides are synthesized from the carbonyl group side (C-terminus) to amino group side (N-terminus) of the amino acid chain in this method, although peptides are synthesised in the opposite direction in cells. In peptide synthesis, an amino-protected amino acid is bound to a solid phase material (most commonly, low cross-linked polystyrene beads), forming a covalent bond between the carbonyl group and the resin, most often an amido or an ester bond. Then the amino group is deprotected and reacted with the carbonyl group of the next amino-protected amino acid. The solid phase now bears a dipeptide. This cycle is repeated to form the desired peptide chain. After all reactions are complete, the synthesized peptide is cleaved from the bead.

The protecting groups for the amino groups mostly used in the peptide synthesis are 9-fluorenylmethyloxycarbonyl group (Fmoc) and t-butyloxycarbonyl (Boc). The Fmoc group is removed from the amino terminus with base while the Boc group is removed with acid.

Many amino acids bear functional groups in the side chain. In order to prevent these functional groups from reacting with the incoming N-protected amino acids, a number of other protecting groups specific for the amino acid to be protected is used. In contrast to BOC and Fmoc groups, these have to be stable over the course of peptide synthesis although they are also removed during the final deprotection of peptides.


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